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Exo III-based fluorescence assay for cocaine detection. (A) Schematic of cocaine detection based on Exo III inhibition of cocaine-bound aptamers (right) relative to the readily-digested unbound aptamer (left). (B) Fluorescence spectra for Exo III-treated (black line) and untreated (red line) samples with or without 250 μM cocaine. (C) Calibration curve with SYBR Green I (SGI). Inset depicts the signal gain from 0 to 10 μM target.
