Table 1.
Enzyme | K d (nM) | Obtained ηss | Ideal ηssa |
---|---|---|---|
WT | 30 ± 3 | 5.0% | 4.9% |
Q407A | 45 ± 6 | 5.6% | 5.5% |
R410A | 71 ± 6 | 1.3% | 5.5% |
R410K | 14 ±1 | 5.1% | 5.0% |
aEstimated by using η1 = 6.7%, η2 = 83%, kx = 0.01 s–1 and respective kexc, as described previously9.