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. 2018 May 2;46(13):6857–6868. doi: 10.1093/nar/gky333

Figure 2.

Figure 2.

(A) Stepwise assembly of the reconstituted RNase P RNP (lanes 1–5) and binding of RNase P proteins to RNase P RNA (lanes 6–9). (B) The addition of Pop4 does not result in a mobility shift unless the Rpp1/Pop5/Pop8 protein subcomplex is already present. (C) The presence of Pop6/Pop7 is required for the structural stability of the reconstituted RNP. (D) RNP assembly with the Rpp1/Pop5 complex substituting for Rpp1/Pop5/Pop8. Lanes 1, 10, 18: RNase P RNA alone; other lanes: electrophoretic mobility shifts upon the addition of protein components as indicated above the gel. Protein components are added to RNase P RNA at a 1:1 molar ratio; the resulting RNP complexes are resolved on a native polyacrylamide gel. RNA is stained with Toluidine Blue. Pop6 and Pop7, as well as Rpp1, Pop5, Pop8 or Rpp1, Pop5 formed subcomplexes and were co-expressed and co-purified together.