Figure 5.
Correlation length and smoothness in direction and magnitude of H2B-tagged GFP in U2OS cells using the WM model. (A) A fluorescence microscopy image of a nucleus expressing H2B-GFP; scale bar is 3 μm. (B) Flow field for and zoomed-in region (right) of the black rectangle; the field is color-coded according to the direction of the displacement. Scale bar is 3 μm (left) and 1 μm (right). (C) Correlation length (top) and smoothness parameter (bottom) calculated from regression of empirical correlation functions over time lag for directional correlation of flow fields. Different colors correspond to different conditions. Shaded error bars correspond to the standard deviation over 19 nuclei per condition. (D) As (C) for the vectors’ magnitude.