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. Author manuscript; available in PMC: 2018 Jul 26.
Published in final edited form as: Acta Biomater. 2016 Jun 1;41:293–301. doi: 10.1016/j.actbio.2016.05.040

Figure 2.

Figure 2

The size of polyplexes formed by self-assembly of enhanced green fluorescent protein (pEGFP) DNA with 447 alone or in combination with PEG-PBAE at various polymer:DNA and 447:PEG-PBAE w/w ratios was measured by (A) Nanosight (NTA) or (B) Zetasizer (DLS). The stability of the polyplexes was tested by sizing them after a 24-h incubation in either sodium acetate or PBS at room temperature. #: Indicates formulation conditions where polyplex aggregation is occurring, leading to unreliable size measurements (low particle concentration by NTA or greater than a micron in size by DLS). (C) The zeta potential of polyplexes. Data are mean ± SD of particle population for NTA and mean ± SD of 3 independent measurements for DLS. Red arrows indicate the four formulations that maintained particle stability and were selected for transfection evaluation.