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. Author manuscript; available in PMC: 2018 Jul 26.
Published in final edited form as: Acta Biomater. 2016 Jun 1;41:293–301. doi: 10.1016/j.actbio.2016.05.040

Figure 3.

Figure 3

Flow cytometry data showing uptake at 4 hrs and transfection efficacy at 2 days post treatment of H446 cells with nine PBAE polyplexes. The efficiency is in terms of percentage of live H446 cells positive for Cy3 (uptake) or EGFP (transfection). Efficacy of ePBAEs is compared to that of polyethylenimine (PEI) 2 w/w. Data are mean ± SD (n=4) (*** p < 0.001 compared to untreated). (B) Cytotoxicity of PBAE polyplexes, quantified by normalizing metabolic activity to untreated cells. Data are mean ± SD (n=3) (*** p < 0.001, ** p < 0.01, * P < 0.05 compared to untreated, red line marks 80% viability). (C) Representative fluorescence microscope images (10×) at 48 h post-treatment of H446 cells transfected with 4 different PBAE polyplexes at 60 w/w and controls. Scale bar is 200 μm for all panels.