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. Author manuscript; available in PMC: 2018 Jul 26.
Published in final edited form as: Blood. 2018 Feb 20;131(15):1639–1653. doi: 10.1182/blood-2017-12-820035

Figure 4. Rescue of combined effects of AC220 and CB839 by α-ketoglutarate.

Figure 4

(A-B) Oxygen consumption rate (OCR) in MV411 (A) and MOLM13 (B) cells treated with vehicle control, AC220 1 nM, CB839 100 nM, AC220 and CB839 combination and AC220/CB839 combination + 4 mM of dimethyl-α-ketoglutarate (αKG) measured using a Seahorse analyzer (for MV411 mean ± s.e.m., n=3, for basal respiration ** P=0.0019 between AC220 versus AC220+CB839, and P= 0.018 between AC220+CB839 versus AC220+CB839+αKG, for maximal respiration *** P=0.0002 between AC220 versus AC220+CB839, and P=0.0224 between AC220+CB839 versus AC220+CB839+αKG, two-way ANOVA with Bonferroni’s multiple comparisons; for MOLM13 cells mean ± s.e.m., n=3, for basal respiration * P=0.0298 between AC220 versus AC220+CB839, and P= 0.0182 between AC220+CB839 versus AC220+CB839+αKG, for maximal respiration between * P=0.02 AC220 versus AC220+CB839, and P=0.0148 between AC220+CB839 versus AC220+CB839+αKG, two-way ANOVA with Bonferroni’s multiple comparisons). (C-D) Relative cytoplasmic ROS levels of MV411 (C) and MOLM13 (D) cells treated as in (A-B) (for MV411 mean ± s.e.m., n=5, * P=0.0195, *** P=0.0008; for MOLM13 mean ± s.e.m., n=6, * P=0.0345, ANOVA with Tukey’s multiple comparisons). (E-F) Apoptosis in MV411 (E) and MOLM13 (F) cells treated as in (A-B) (for MV411 mean ± s.e.m., n=10, * P=0.0158 between AC220 versus AC220+CB839 and * P=0.0102 between AC220+CB839 versus AC220+CB839+αKG; for MOLM13 mean ± s.e.m., n=9, * P=0.0105 between AC220 versus AC220+CB839, and * P=0.0120 between AC220+CB839 versus AC220+CB839+αKG, ANOVA with Tukey’s multiple comparisons).