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. Author manuscript; available in PMC: 2018 Jul 26.
Published in final edited form as: Biochem Biophys Res Commun. 2017 May 4;488(1):129–135. doi: 10.1016/j.bbrc.2017.05.021

Fig. 2. Characterization of proteins and complexes by SDS-PAGE and SEC.

Fig. 2.

(A) Mdm12 proteins. Sce, Scas and Ddis together with the Sce-Mdm12T4L internal fusion protein. The two Ddis-Mdm12 peaks correspond to a dimer/monomer mixture, all other proteins are monomeric. (B) Mdm12/Mmm1Δ heterotetrameric complexes. Scas-Mdm12 and Sce- Mmm 1Δ have identical molecular weight and cannot be resolved on this gel. For Scas-Mdm12/Sce-Mmm1Δ complex, excess of free monomeric Scas-Mdm12 is separated from the complex. (C) Crystals of Mdm12/Mmm1Δ complexes.