Fig. 2. The expression and epigenetic profiling of Pdx1 at birth. (A) The expression level of mRNA for Pdx1 was determined at birth (n = 6). (B) The abundance of acetylated H3 (AceH3), acetylated H4 (AceH4), H3K4me3, and H3K9me2 at the Pdx1 promoter (n = 3). Data are represented as percent of input control, which undergo analyses exactly as the test samples except for the IP step. (C) A schematic illustration of the analyzed CpG sites at the Pdx1 proximal promoter. CpG sites are marked with open circles relative to the translational start site (+1 bp). (D) DNA methylation status of the Pdx1 promoter (n = 3). Data are represented as mean ± SEM. *p < 0.05, **p < 0.01; ***p < 0.001 by two-tailed Student's t-test.