Fig. 2. Increased yield of DNA double-strand breaks by uranyl nitrate. (A) Effect of different concentrations of uranyl nitrate on the expression of γH2AX protein. The γH2AX protein level was detected by the western blot in BEP2D after treatment with 0, 1, 5, 10, 20 and 40 μM of uranyl nitrate for 24 h. The expression intensities were quantified using the Quantity One software. β-Actin was used as a loading control. (B) Effect of different treatment times of uranyl nitrate on γH2AX protein expression. (C) The intensities of γH2AX expression western blot bands were quantified using the Quantity One software. β-Actin was used as a loading control, and the data of ratio were standardized by control. (D) Comet images of DSBs detected by neutral cell gel electrophoresis. (E) The statistical histogram of comet tail moment. *P < 0.05, **p < 0.01.