Table 4. Gene expression analysis of xenobiotic nuclear receptors and their target genes in a mouse model of NAFLD ^a .

Genes	Fold change over control by qPCR	Fold change over control by RNA-seq b
Pparα	2.27	1.35
Pparγ	2.24	1.72
Pparδ/β	–3.85	–2.02
Cyp4a10	2.58	1.25
Car (Nr1i3)	1.48	1.72
Pxr (Nr1i2)	1.56	1.23
Cyp2b10	21.92	3.27
Cyp3a11	4.20	1.83
Ahr	2.30	1.42
Cyp1a1	1.76	1.36
Cyp1a2	–1.69	–1.86
Lxrα	–1.02	–1.11
Abcg5	4.08	2.42
Abcg8	3.58	2.06
Fxr	1.14	1.12
Abcb4	2.12	1.89
Abcb11	1.11	–1.36

^aFatty and normal liver samples were generated from C57BL/6 mice fed with western diet or low-fat control diet for 16 weeks, respectively.

^bDifferential expression analysis was done using DESeq-2 package in R. Bold: Statistically different from controls (for qPCR analysis: N = 4–6; student two-tailed t-test, p < 0.05; for RNA-seq: N = 4; fold change >1.5; adjusted p < 0.05).