Table 5. Ingenuity pathway analysis predicts hepatic xenobiotic nuclear receptor activation in a mouse model of NAFLD ^a .

Upstream nuclear receptor	Predicted activity	Activation z-score	p-Value
PPARα	+++	4.159	1.08 × 10–50
PPARγ	+	1.342	5.73 × 10–15
PPARδ	+++	2.815	9.53 × 10–15
CAR	+	0.972	1.71 × 10–13
PXR	+++	3.153	1.13 × 10–19
AHR	-	-1.740	3.67 × 10–16
LXR	+	0.886	8.89 × 10–9
FXR	+	0.943	1.41 × 10–10

^aSteatoic and normal liver samples were generated from C57BL/6 mice fed with western diet for 16 weeks. RNA-seq (4 samples per group) was done to profile the whole transcriptome of these samples. Differential expression analysis was done using DESeq-2 package in R. Ingenuity pathway analysis was done to predict the activation of xenobiotic nuclear receptors based on differential expression analysis results. Activation z-score and p-value were calculated by the build-in algorithms in IPA.