Table 2. List of primers of the inflammatory mediators in qRT-PCR analysis.
Gene symbol | Accession number | Primer | Primer sequence (5′–3′) | Product size | T m (°C) |
NF-κB | NM205134 | Forward | CTGAAACTACTGATTGCTGCTGGA | 179 bp | 62 |
Reverse | GCTATGTGAAGAGGCGTTGTGC | ||||
IFN-γ | Y07922 | Forward | AGCTGACGGTGGACCTATTATT | 157 bp | 58 |
Reverse | GGCTTTGCGCTGGATTC | ||||
TNF-α | NM204267 | Forward | CCCCTACCCTGTCCCACAA | 100 bp | 58 |
Reverse | TGAGTACTGCGGAGGGTTCAT | ||||
COX-2 | NM001167718 | Forward | CTTAAATTGAGACTTCGCAAGGATG | 165 bp | 62 |
Reverse | TGGGACCAAGCCAAACACCT | ||||
iNOS | NM204961 | Forward | CCGTGTTCCACCAGGAGATG | 168 bp | 56 |
Reverse | GCAGGAGTAATGACGCCAAGAG | ||||
IL-1β | Y15006 | Forward | CAGCCTCAGCGAAGAGACCTT | 106 bp | 60 |
Reverse | CACTGTGGTGTGCTCAGAATCC | ||||
IL-2 | AF000631 | Forward | TCTGGGACCACTGTATGCTCT | 138 bp | 60 |
Reverse | ACACCAGTGGGAAACAGTATCA | ||||
IL-4 | AJ621249 | Forward | ACCCAGGGCATCCAGAAG | 176 bp | 59 |
Reverse | CAGTGCCGGCAAGAAGTT | ||||
IL-6 | AJ309540 | Forward | AATCCCTCCTCGCCAATCTG | 106 bp | 60 |
Reverse | GCCCTCACGGTCTTCTCCATA | ||||
IL-8 | HM179639 | Forward | CTGGCCCTCCTCCTGGTT | 105 bp | 60 |
Reverse | GCAGCTCATTCCCCATCTTTAC | ||||
IL-13 | AJ621250 | Forward | AGTGCTGGACAACATGACCGA | 128 bp | 60 |
Reverse | GCAAGAAGTTCCGCAGGTAGAT | ||||
IL-18 | AJ277865 | Forward | TAGCCAGTTGCTTGTGGTTCG | 170 bp | 60 |
Reverse | TCTTATCTTCTACCTGGACGCTGA | ||||
β-Actin | L08165 | Forward | TGCTGTGTTCCCATCTATCG | 178 bp | 62 |
Reverse | TTGGTGACAATACCGTGTTCA |