. 2016 Jul 8;5(5):1421–1433. doi: 10.1039/c6tx00197a

Table 2. List of primers of the inflammatory mediators in qRT-PCR analysis.

Gene symbol	Accession number	Primer	Primer sequence (5′–3′)	Product size	T _m (°C)
NF-κB	NM205134	Forward	CTGAAACTACTGATTGCTGCTGGA	179 bp	62
NF-κB	NM205134	Reverse	GCTATGTGAAGAGGCGTTGTGC	179 bp	62
IFN-γ	Y07922	Forward	AGCTGACGGTGGACCTATTATT	157 bp	58
IFN-γ	Y07922	Reverse	GGCTTTGCGCTGGATTC	157 bp	58
TNF-α	NM204267	Forward	CCCCTACCCTGTCCCACAA	100 bp	58
		Reverse	TGAGTACTGCGGAGGGTTCAT
COX-2	NM001167718	Forward	CTTAAATTGAGACTTCGCAAGGATG	165 bp	62
		Reverse	TGGGACCAAGCCAAACACCT
iNOS	NM204961	Forward	CCGTGTTCCACCAGGAGATG	168 bp	56
iNOS	NM204961	Reverse	GCAGGAGTAATGACGCCAAGAG	168 bp	56
IL-1β	Y15006	Forward	CAGCCTCAGCGAAGAGACCTT	106 bp	60
IL-1β	Y15006	Reverse	CACTGTGGTGTGCTCAGAATCC	106 bp	60
IL-2	AF000631	Forward	TCTGGGACCACTGTATGCTCT	138 bp	60
IL-2	AF000631	Reverse	ACACCAGTGGGAAACAGTATCA	138 bp	60
IL-4	AJ621249	Forward	ACCCAGGGCATCCAGAAG	176 bp	59
IL-4	AJ621249	Reverse	CAGTGCCGGCAAGAAGTT	176 bp	59
IL-6	AJ309540	Forward	AATCCCTCCTCGCCAATCTG	106 bp	60
IL-6	AJ309540	Reverse	GCCCTCACGGTCTTCTCCATA	106 bp	60
IL-8	HM179639	Forward	CTGGCCCTCCTCCTGGTT	105 bp	60
IL-8	HM179639	Reverse	GCAGCTCATTCCCCATCTTTAC	105 bp	60
IL-13	AJ621250	Forward	AGTGCTGGACAACATGACCGA	128 bp	60
		Reverse	GCAAGAAGTTCCGCAGGTAGAT
IL-18	AJ277865	Forward	TAGCCAGTTGCTTGTGGTTCG	170 bp	60
		Reverse	TCTTATCTTCTACCTGGACGCTGA
β-Actin	L08165	Forward	TGCTGTGTTCCCATCTATCG	178 bp	62
β-Actin	L08165	Reverse	TTGGTGACAATACCGTGTTCA	178 bp	62