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. 2018 Jul 26;13(7):e0201231. doi: 10.1371/journal.pone.0201231

Fig 4. Contribution of Rho effector proteins and associated proteins to the response to thrombin.

Fig 4

A—Quantification of the area under the response curve (AUC) of HUVECs transfected with 80 different siRNAs targeting Rho-effector proteins (and associated genes) stimulated with 1U/ml of thrombin for 3h at 72h post-transfection (n = 3±SD). In blue, on the left, NT control siRNA (n = 18, in triplicate), in red Rho-effector proteins with a significantly different AUC compared to NT control siRNA and in green, siMLC1. (Color code refers to the change in AUC, see S1A–S1C Fig) ****P<0.0001 in two-tailed Student’s T-test and FDR<0.05; B-G—Normalized endothelial resistance of HUVECs transfected with the indicated siRNAs and stimulated with 1U/ml of thrombin for 3h at 72h post-transfection (n = 3±SD); Arrow indicates the moment at which thrombin was added.