Table 1. List of novel and known mediators of thrombin–induced endothelial response identified in the functional siRNA screen.
| siRNA Target | AUC | 2-tailed T-test p-value | FDR |
|---|---|---|---|
| RTKN | -1.267±0.04 | 0.00000457 | 0.000157 |
| TIAM2 | -1.017±0.05 | 0.0000116 | 0.001013 |
| MLC1* | -1.145±0.02 | 0.0000115 | 0.001013 |
| ARPC1B | -1.304±0.02 | 0.0000117 | 0.001013 |
| SEPT2 | -1.065±0.03 | 0.0000174 | 0.001097 |
| SLC9A3R1 | -0.972±0.05 | 0.0000191 | 0.001097 |
| RACGAP1 | -1.025±0.05 | 0.0000344 | 0.001686 |
| RAPGEF2* | -1.190±0.06 | 0.0000508 | 0.001945 |
| RHOD | -0.993±0.06 | 0.0001203 | 0.003765 |
| PREX1* | -0.815±0.04 | 0.0001493 | 0.004281 |
| ARHGEF7* | -1.041±0.08 | 0.0002136 | 0.004806 |
| PLXNB2 | -0.914±0.06 | 0.0001869 | 0.004806 |
| ARHGAP45* | -0.924±0.05 | 0.0002235 | 0.004806 |
| SRGAP2 | -0.902±0.04 | 0.0002055 | 0.004806 |
| ARHGEF5 | -0.942±0.10 | 0.0022917 | 0.039419 |
Overview of the genes identified in the siRNA screen as significant hits and ranked by False Discovery Rate (FDR) values. Area under the response curve (AUC) determined upon stimulation with 1U/ml of thrombin (n = 3 ±SD) and T-test p-values.
*Known genes identified in this screen related to thrombin-induced signaling in endothelial cells.