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. 2018 Jul 26;13(7):e0201250. doi: 10.1371/journal.pone.0201250

Table 1. Virus titera in supernatant fluids collected at 5 days post-transfection (dpt) and at the time of post-transfection showed a strong CPE for each cDNA construct.


Construct
Titer (pfu/ml)
measured at 5 dptb
Sampling time when
showed strong CPE
Titer (pfu/ml) measured at
the time showed strong CPEc
WT 8.50 × 105 4 dpt 1.35 × 106
Δ307 0 11 dpt 0
ΔAU-rich 0 9 dpt 1.90 × 105
Δ5’-SL 1.55 × 105 6 dpt 9.75 × 105
ΔCYC-like 3.05 × 103 6 dpt 4.50 × 105
ΔSLII 0 10 dpt 5.58 x 105
ΔSLII.1 0 8 dpt 3.65 × 105
ΔSLII.2 0 8 dpt 4.40 × 105
ΔSLII.3 3.25 × 105 6 dpt 5.15 × 105
PK1’ 0 10 dpt 2.48 × 104
PK1” 2.35 × 105 6 dpt 4.70 × 105
PK1’1” 3 × 104 6 dpt 5.25 × 105

aVirus assays on BHK-21 cells using an agarose overlay.

bCells were passaged at five days post-transfection (dpt) and supernatant fluids were collected at the time of passaging.

cSampling time for measuring the titer is indicated on the left column.