Table 1. Virus titera in supernatant fluids collected at 5 days post-transfection (dpt) and at the time of post-transfection showed a strong CPE for each cDNA construct.
Construct |
Titer (pfu/ml) measured at 5 dptb |
Sampling time when showed strong CPE |
Titer (pfu/ml) measured at the time showed strong CPEc |
---|---|---|---|
WT | 8.50 × 105 | 4 dpt | 1.35 × 106 |
Δ307 | 0 | 11 dpt | 0 |
ΔAU-rich | 0 | 9 dpt | 1.90 × 105 |
Δ5’-SL | 1.55 × 105 | 6 dpt | 9.75 × 105 |
ΔCYC-like | 3.05 × 103 | 6 dpt | 4.50 × 105 |
ΔSLII | 0 | 10 dpt | 5.58 x 105 |
ΔSLII.1 | 0 | 8 dpt | 3.65 × 105 |
ΔSLII.2 | 0 | 8 dpt | 4.40 × 105 |
ΔSLII.3 | 3.25 × 105 | 6 dpt | 5.15 × 105 |
PK1’ | 0 | 10 dpt | 2.48 × 104 |
PK1” | 2.35 × 105 | 6 dpt | 4.70 × 105 |
PK1’1” | 3 × 104 | 6 dpt | 5.25 × 105 |
aVirus assays on BHK-21 cells using an agarose overlay.
bCells were passaged at five days post-transfection (dpt) and supernatant fluids were collected at the time of passaging.
cSampling time for measuring the titer is indicated on the left column.