Fig 1. Micropatterning via photolithography resulted in cultured monolayers of Ex293 cells around acellular regions that varied in size and spacing.

(a) Homogenous monolayer; obstacle-to-strand ratio: 0; (b) 150 μm x 150 μm obstacles, separated by 100 μm strands; obstacle-to-strand ratio: 1.5; (c) 700 μm x 700 μm obstacles, separated by 100 μm strands; obstacle-to-strand ratio: 7.0 (scale bar = 150 μm); (d) Schematic depiction of tissue obstacle structure; (e) Obstacle and strand widths, and obstacle percent density, for each degree of heterogeneity.