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. 2018 Jul 26;13(7):e0201453. doi: 10.1371/journal.pone.0201453

Fig 1. The experiment-timeline design.

Fig 1

The picture of the rat head represents the parameters used for tumor induction: 10µL of C6Luc solution were administered during 10 min, according to the following coordinates: AP 2 mm; LL 2 mm; D 2.5 mm from bregma. The experimental groups were divided as control, sham and tumor. In vivo experiments comprised behavioral tests, BLI, and MRI analysis, using the same animals of each group to perform all timepoints (X) (4 rats per group, totalizing 12 rats). Ex vivo experiments comprised BLI and histological analysis. A new group of 48 rats was used (4 rats per each group and each time point) until the 21st day after the induction (O). 1 rat per group/time was used in the BLI analysis, and the same group of rats was used in the BLI analysis to complete the number of 4 rats per group/ time used for histological analysis. The “X” into the red square represents that the same 12 animals of the in vivo experiments were used in the last time point of the ex vivo experiments (28th day).