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. 2018 Jul 16;14(7):e1007500. doi: 10.1371/journal.pgen.1007500

Fig 10. miR-210 modulates the expression of multiple genes.

Fig 10

Luciferase assays were performed 24 hours post-transfection on S2R+ cells transfected with miR-210 precursor (pUAST-miR-210 + p-Act-Gal4) or negative controls (only p-Act-Gal4). (A) Luciferase relative levels in cells transfected with reporter constructs containing wild-type or mutant 3’-UTRs for the indicated genes, or a synthetic sequence including 3 perfect miR-210 binding sites (sensor). Results from three independent experiments are shown as mean ± SD of firefly luciferase activity relative to controls. t test; *p<0.05; **p<0.01; ***p<0.005; ns, not significant. (B) Reporter constructs containing miR-210 wild type or mutant binding sites in mnd, SoxN, Bsg, scrib and 3’-UTRs (sensor). mut, mutation; del, deletion.