Fig. 1. Experimental procedure and biological endpoints tested. Upper panel illustrates the smoking machines and Vitrocell® 24/48 exposure system used for exposing cultures to 3R4F smoke or THS2.2 aerosol. Lower left panel illustrates 3D organotypic cultures and the various biological endpoints assessed in the study. Lower right panel shows the number of experimental repetitions conducted for 3R4F smoke and THS2.2 aerosol exposure.
