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. 2017 Apr 7;6(4):e16241. doi: 10.1038/lsa.2016.241

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Working principles of the R-TPM approach. (a) Sketch of the experimental R-TPM set-up. Cells are injected into a microfluidic channel and tumble while flowing along the y-axis (inset of a). At the same time, a holographic image sequence is acquired. In the top-left corner of the inset, the reference system for cell tumbling is reported; in the top-right corner, a photo of the real set-up is shown. Rotation occurs around the x and z axes. BS, beam splitter; DM, dichroic mirror; MC, microchannel. (b) Flow chart representing the main steps of the two proposed algorithms for angle recovery and tomographic reconstruction.