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. Author manuscript; available in PMC: 2019 Aug 1.

Published in final edited form as: J Am Soc Mass Spectrom. 2018 Jun 12;29(8):1745–1756. doi: 10.1007/s13361-018-1999-6

Comparison of extracted ion chromatograms (XICs) for Ψ, t⁶A, oQ and k²C detected in E. coli using (a) LC method 1 and (b) LC method 2. * indicates t⁶A diastereomer. Corresponding HCD fingerprints from each of the different LC methods for Ψ (c) method 1/(d) method 2, t⁶A (e) method 1/(f) method 2, oQ (g) method 1/(h) method 2 and k²C (i) method 1/(j) method 2.

Comparison of extracted ion chromatograms (XICs) for Ψ, t⁶A, oQ and k²C detected in E. coli using (a) LC method 1 and (b) LC method 2. * indicates t⁶A diastereomer. Corresponding HCD fingerprints from each of the different LC methods for Ψ (c) method 1/(d) method 2, t⁶A (e) method 1/(f) method 2, oQ (g) method 1/(h) method 2 and k²C (i) method 1/(j) method 2.