Table 1.
RIAs of the non-unique ions with m/z 126.0662, 109.0307, 108.0557, 83.0605, 82.0288, 81.0448, 56.0494, and 54.0337 observed in the HCD MS/MS spectra of m3C, m4C and m5C. Mean and RSD were calculated by accounting data acquired intraday though the analysis of replicates (n=5), and interday (2 different days) where different amount of nucleoside (i.e., 5 and 20 ng) and different LC conditions (i.e., LC method 1 and 2) were employed for the analysis (n=20). m/z represented are based on theoretical calculations.
|
|
||||||||
|---|---|---|---|---|---|---|---|---|
| Intraday (n=5) | Interday and different conditions (n=20) | |||||||
|
| ||||||||
| m3C | m4C | m5C | m3C | m4C | m5C | |||
|
|
||||||||
| Non-unique ion m/z | 126.0667 | 100 ± 0.5 | 100 ± 0.0 | 100 ± 0.0 | 99 ± 2.3 | 100 ± 0.0 | 100 ± 0.0 | |
| 109.0402 | 97 ± 3.5 | 62 ± 6.2 | 85 ± 6.2 | 98 ± 2.6 | 63 ± 5.8 | 83 ± 4.6 | ||
| 108.0562 | 2.3 ± 19 | 63 ± 4.1 | 36 ± 8.6 | 2.2 ± 19 | 63 ± 4.4 | 36 ± 5.5 | ||
| 83.0609 | 6.1 ± 7.5 | 47 ± 5.0 | 45 ± 7.0 | 6.3 ± 6.9 | 46 ± 5.8 | 44 ± 5.6 | ||
| 82.0293 | 4.8 ± 10 | 3.4 ± 8.2 | 9.5 ± 15 | 4.7 ± 16 | 3.3 ± 13 | 9.9 ± 11 | ||
| 81.0453 | 3.4 ± 10 | 7.4 ± 9.7 | 26 ± 11 | 3.3 ± 16 | 7.2 ± 11 | 26 ± 9.9 | ||
| 56.0500 | 2.9 ± 8.5 | 29 ± 7.0 | 16 ± 12 | 2.5 ± 18 | 29 ± 8.8 | 16 ± 10 | ||
| 54.0344 | 1.5 ± 18 | 2.1 ± 29 | 9.5 ± 17 | 1.7 ± 26 | 2.1 ± 25 | 8.8 ± 14 | ||
|
|
||||||||