Fig. 2. Identification of eIF4G1 and its HtrA2/Omi linked cleavage products by western blots in SH-SY5Y cells. (A) Cells were treated with DMSO (0.25% for 12 h), cisplatin (30 mg ml^–1 for 16 h), camptothecin (86 nM for 16 h), doxorubicin (5 μM for 8 h), dicumarol (300 μM for 10 h), etoposide (20 μM for 8 h), or H₂O₂ (100 μM for 6 h). Lysates were analyzed by western blot for the endogenous levels of native eIF4G1 protein (220 kDa) and the 45 kDa HtrA2/Omi linked cleavage product with a monoclonal eIF4G1 antibody (Abcam), or for the indicated proteins. An asterisk indicates non-specific binding (B) Protease inhibitor treatment does not change the expression level of HtrA2/Omi substrate proteins. SH-SY5Y cells were exposed to 0.25% DMSO, BTZ or CFZ for 12 hours. Whole cell extracts were analyzed by western blots for the endogenous levels of the indicated proteins. Results are representatives of two independent experiments.