Fig. 3. Cleavage of eIF4G1 and UCH-L1 in mouse embryonic fibroblasts is HtrA2/Omi dependent. (A) Wild type MEF cells were treated with 500 nM staurosporine (Stauro.) for 8 h and/or ucf-101 for 6 h. (B) Wild type (htra2 +/+) and HtrA2/Omi-deficient (htra2 –/–) mouse embryonic fibroblast (MEF) cells were treated with staurosporine, 86 nM camptothecin, or 20 μM etoposide. Endogenous level of full length eIF4G1, UCH-L1, HtrA2/Omi, and GAPDH was assayed by western blots in cell extracts. (C) Wild type and HtrA2/Omi-deficient MEFs were treated with 500 nM staurosporine or ucf-101 for 8 hours.
