Table 1. Sequences of primers used in the real-time qPCR amplifications.
Target gene | Primer sequences (5′–3′) | Length of PCR product (bp) |
β-tublin | F: TGTGGCAACCAGATCGGT | 204 |
R: ACCTGAGCGAACAGAGTCCA | ||
p53 | F: TTCCACGACGGTGACACG | 253 |
R: TGGGAGCTTCATCTGGACCT | ||
p21 | F: TTAGCAGCGGAACAAGGAGT | 209 |
R: CCAGGCCAGTATGTTACAGGA | ||
Cdk2 | F: ATCCGCCTGGACACTGAG | 273 |
R: GTGGAGGACCCGATGAGA | ||
CyclinE | F: TTGGCTATGCTGGAGGAAGT | 116 |
R: CCTGGTGGTTTTTCAGTGCT | ||
p27 | F: TGCAACCGACGATTCTTCTACTCAA | 185 |
R: CAAGCAGTGATGTATCTGATAAACAAGGA |