Fig. 7. Immunofluorescence analysis of intracellular localization of red emitting NCs. HeLa cells treated with cationic PC/DOTAP-liposomes for 15 min or 1 h were fixed and subjected to immunofluorescence analysis using anti-TfR (A) and anti-LAMP-1 (B) antibodies, as indicated, followed by FITC-secondary antibodies (green). For each image, magnifications of the boxed areas are shown in the respective lower insets. (C) 3D surface plots of the images presented in A. (D) 3D surface plots of the images presented in B. Experiments were conducted with 40 μg mL–1 of NC-liposomes containing 20 nM DDT-capped CdSe@ZnS NCs.
