Fig. 4. Role of SIRT1 in MTBE-induced oxidative stress. Four oxidative stress indexes, including ROS (A–B), MDA (C), GSSG (D), SOD (E), and the ratio of GHS/T-GSH (F) were detected in HT22 cells pretreated with SRT1720 for 2 h and HT22 cells transfected with siRNA-SIRT1, after exposure to 0 (Con), 0.125, 0.25, 0.5, 1.0 and 2.0 mM MTBE for 6 h, respectively. (A) was captured by a fluorescent microscope under 200× magnification. Data are expressed as mean ± SD. In siRNA-SIRT1 HT22 cells, *P < 0.05 versus control, and ^#P < 0.05 versus NC (empty lentiviral vector); while in SRT1720 HT22 cells, **P < 0.05 versus control, and ^##P < 0.05 versus NC (not pretreated with SRT1720).