Fig. 5. Detection of MALAT1 changes in gene mRNA expression during cadmium-induced malignant transformation of 16HBE cells. Untreated control 16HBE cells, Cd-transformed 15th passage cells and 35th passage cells were independently treated with MALAT1-siRNA, the mRNA expression of FOXC2, STAT3, BCL-2, BAX, EGFR and TGF-β1 genes were detected after 72 h by qPCR. The threshold cycle number (Ct value) for each gene obtained with qPCR was normalized to Ct value of β-actin from the same sample, and fold changes in expression for each gene were obtained with the delta–delta Ct method. The graph shows the mean ± SD of triplicate values. *P < 0.05, compared with 16HBE cells; #P < 0.05, compared with the control group of the same passage cells (untreated with si-MALAT1) (one-way ANOVA).
