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. 2017 Aug 24;6(6):878–888. doi: 10.1039/c7tx00126f

Fig. 6. The miR-29b inhibited the activation of MRC-5 cells induced by supernatants from silica-treated macrophages. (a) Transfection efficiency for MRC-5 cells checked by examination of Cy3 using an Eclipse 80i microscope after 24 h incubation. The Cy3 signals showed that mimics were distributed in keeping with cell shape. (b, c, d) qRT-PCR analyses of mRNA expression levels of collagen1, collagen4 and fibronectin, respectively. mRNA levels were increased in the silica 50 μg mL–1 group, while they were decreased in the silica 50 + mimics group. (e, f) Western blotting results of the protein expression levels of collagen3. The protein level was increased in the silica 50 μg mL–1 group while it was decreased in the silica 50 + mimics group. The data are presented as the means ± SD (n = 3). The significance of differences was determined by ANOVA followed by the Dunnett's test; ^p < 0.05 versus the silica 0 group, ^^p < 0.01 versus the silica 0 group, #p < 0.05 versus the silica 50 group, ##p < 0.01 versus the silica 50 group.

Fig. 6