Fig. 3. Quantification of epithelial, mesenchymal markers and BMP-7 protein expression in RLE-6TN cells exposed to silica. Representative western blots (n = 3) and densitometric analysis demonstrated decreases in the expression of E-cadherin (A and B), CK-19 (A) and BMP-7 (A and E); increases in vimentin (A and C) and α-SMA (A and D) in RLE-6TN cells treated with the increase of silica for 48 h. GAPDH was used as a loading control for all blots, and densitometric analysis was adjusted to account for relative differences in GAPDH intensity. *P < 0.05 vs. control.