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. 2015 Nov 18;5(1):278–290. doi: 10.1039/c5tx00187k

Table 3. Antibodies and their concentrations employed in Western blotting and immunohistochemical staining.

Antigen/specificity Mwt (kDa) Dilution Antibodies and source
β-Actin 44 1 : 4000 WB Mouse monoclonal IgG (Sigma Aldrich A5441)
Albumin 60 1 : 3000 WB Chicken polyclonal to liver albumin (abcam ab106582)
1 : 400 IHC(P)
Amylase 57 1 : 3000 WB Rabbit polyclonal to pancreatic amylase (abcam ab21156)
1 : 100 IHC(P) Mouse monoclonal (Santa Cruz sc-46657)
CEBP-β 36 1 : 100 IHC(P) Rabbit polyclonal to CEBP Beta (abcam ab53138)
CK18 48 1 : 100 IHC(P) Rabbit monoclonal to Cytokeratin 18 (abcam ab133263)
CPS-1 165 1 : 2000 WB Rabbit polyclonal to CPS1 – Liver Mitochondrial Marker (abcam ab3682)
CYP2E1 50 1 : 5000 WB Rabbit polyclonal to CYP2E1 (abcam ab28146)
1 : 100 IHC(P) Rabbit polyclonal to CYP2E1 (abcam ab73878)
CYP3A4 58 1 : 2000 WB Rabbit polyclonal to CYP3A4 (abcam ab155029)
MRP2 190 1 : 100 IHC(P) Rabbit polyclonal to MRP2 (Sigma Aldrich M8316)
Anti-chicken 2° N/A 1 : 1000 IHC(P) Alexa-Fluor®-594 goat anti-chicken IgG (H + L) (life technologies A-11042)
Anti-mouse 2° N/A Alexa-Fluor®-488 goat anti-mouse IgG (H + L) (life technologies A-11029)
Anti-rabbit 2° N/A Alexa-Fluor®-594 goat anti-rabbit IgG (H + L) (life technologies A-11037)
Anti-goat HRP 2° N/A 1 : 3000 WB Polyclonal to goat produced in rabbit, horseradish peroxidise conjugated (Sigma Aldrich A5420)
Anti-rabbit HRP 2° N/A Polyclonal to rabbit produced in goat, horseradish peroxidise conjugated (Dako P0448)
Anti-mouse HRP 2° N/A Polyclonal to mouse produced in goat, horseradish peroxidise conjugated (Dako P0447)