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Fig. 5. Real-time heat detection of mitochondria by microcalorimetry. A low concentration of Ag+ (2–20 μM) has little impact on mitochondria (3.5 mg mL–1) (A), while higher concentration (50 and 100 μM) does obviously inhibit caloric metabolization, decreasing the pmax, decelerating the heat release and delaying the tmax (B). However the total heat (Q) doesn't change much because of a certain amount of air in the ampoules.