Fig. 1. Summary of study design for this research. The effects of phenobarbital (PB) on DNA methylation (5mC), DNA hydroxymethylation (5hmC) and gene expression were determined in hepatocellular adenomas induced by DEN/500 ppm PB. Then the altered genes detected in the above analysis were compared to those altered at an early phase of PB treatment in the liver of wild-type mice and chimeric mice with human hepatocytes.39.
