Table 1. Multi-parametric cellular phenotypic assay panel.
| Assay and parameters | Fluorescent probes |
| 1. Cellular morphology and cytoskeletal assay | |
| • Nuclear size and intensity | • Hoechst 33342 a |
| • F-actin | • Phalloidin (Alexa 488) |
| • α-Tubulin | • α-Tubulin Ab (Alexa 546) |
| • Cell morphology | • CellMask Deep Red |
| 2. Cytotoxicity assay 1 | |
| • Cell count | • Hoechst 33342 |
| • MnSOD content | • MnSODAb (Alexa 488) |
| • Mitochondrial membrane potential and mass (MMP) | • MitoTracker Red CMXRos |
| • Nuclear membrane permeability (NMP) | • TOTO-3 |
| 3. Oxidative stress assay | |
| • Cell count | • Hoechst 33342 |
| • Reduced glutathione (GSH) | • mBCI |
| • Reactive oxygen species (ROS) | • CM-H2DCFDA |
| 4. Cytotoxicity assay 2 | |
| • Cell count | • Hoechst33342 |
| • Autophagy | • LC3B Ab (Alexa 488) |
| • Lysosome membrane potential and mass | • LysoTracker Red |
| • Phosphohistone H2AX, Ser139 (DNA damage) | • pH2AXAb (Alexa 647) |
| 5. Apoptosis & necrosis assay | |
| • Cell count | • Hoechst 33342 |
| • Early apoptosis | • AnnexinV (Alexa 488) |
| • Late apoptosis | • PI |
| • Necrosis | |
| 6. Cell cycle arrest assay | |
| • Cell count | • Hoechst 33342 |
| • Cell cycle disruption | |
aThe colors of the probes represent the differential fluorescent colors of probes at the specified emission wavelength.