Table 2. Output parameters and their implications.
| Assay targets | Parameters | Implications | Z′ factor |
| Assay 1 | |||
| Nuclear a (Nuc) | Cell count | Number of cells used in the assay | 0.66 |
| Nuc intensity | Mean nuclear intensity | 0.48 | |
| Nuc area | Area of the identified nucleus | 0.73 | |
| Nuc elongation | Ratio of the short axis to the long axis of the nucleus | — | |
| Nuc 1/(form factor) | Mean roundness index of the nucleus, perimeter 2/(4π × area) | 0.46 | |
| Nuc intensity CV | Coefficient of variation of the intensity of pixels over the population of pixels comprising the nuclear region | 0.62 | |
| F-Actin | Intensity | Average intensity of pixels for the filaments | 0.47 |
| 1/(form factor) | Mean roundness index of actins, perimeter 2/(4π × area) | — | |
| Elongation | Mean ratio of the short axis to the long axis of the filament | — | |
| Total area | Total area of filaments | 0.53 | |
| α-Tubulin | Intensity | Average intensity of pixels for tubulin | 0.46 |
| 1/(form factor) | Mean roundness index of tubulin, perimeter 2/(4π × area) | — | |
| Elongation | Mean ratio of the short axis to the long axis of tubulin | — | |
| Total area | Total area of tubulin | 0.60 | |
| Total-cell shape | Intensity × total area | Average content of tubulin in cells | — |
| 1/(form factor) | Area of cells | 0.45 | |
| Elongation | Mean intensity of pixels within the cell | — | |
| Total area | Ratio of the short axis to the long axis of the cell | 0.45 | |
| Cell 1/(form factor) | Cell roundness index, perimeter 2/(4π × area) | — | |
| Cell intensity CV | Coefficient of variation of pixel intensities over the population of pixels in the cell region | 0.56 | |
| Assay 2 | |||
| MnSOD | Intensity × total area | Average content of MnSOD in cells | 0.87 |
| MMP | Intensity × total area | Average level of MMP in cells | 0.80 |
| NMP | Intensity | Average intensity of TOTO-3 pixels within the nucleus | 0.48 |
| Assay 3 | |||
| GSH | Intensity × total area | Average content of GSH in cells | 0.46 |
| ROS | Intensity × total area | Average content of ROS in cells | 0.86 |
| Assay 4 | |||
| LC3B | Intensity × total area | Average content of LC3B in cells | 0.47 |
| Lysosome | Intensity × total area | Average number of lysosomes in cells | 0.56 |
| pH2AX | Intensity × total area | Average content of pH2AX within the nucleus | 0.65 |
| Assay 5 | |||
| mPLextro. | Intensity × total area | Average level of early apoptosis | 0.78 |
| mPLextro + Nuc(PI) | Intensity × total area | Average level of late apoptosis | 0.93 |
| Nuc(PI) | Intensity × total area | Average level of Necrosis | 0.81 |
| Assay 6 | |||
| Cell cycle | Intensity and Area | G0–G1; S; G2; M | >0.7 |
aNuclear analysis was included in each assay. mPLextro, membrane phospholipid extroversion; Nuc(PI), nuclei positively stained with propidiumiodide.