Table 2.
Primary antibodies utilized for immunoblots and immunofluorescence
| Target | Species | Company | Catalog # | Immunoblot conditions | IF 1° antibody dilution |
|---|---|---|---|---|---|
| TLR4 | rabbit | Abcam | 13556 | NA | 1:50 ON |
| TLR4 | rabbit | SCBT | 10741 | 200 μg, 1:2000 ON | NA |
| TLR5 | mouse | Novus | NBP2-24787 | 200 μg, 1:500 ON | 1:50 ON |
| TLR6 | rabbit | Rockland | 600-401-FG3 | 300 μg, 1:500 ON | 1:100 ON |
| TLR7 | rabbit | Rockland | 200-401-A64 | 275 μg, 1:500 ON | 1:100 ON |
| Vimentin | rabbit | SCBT | 5565 | NA | 1:200 ON |
| Vimentin | mouse | SCBT | 373717 | NA | 1:200 ON |
| Calretinin | rabbit | SCBT | 50453 | NA | 1:50 ON |
| Calretinin | mouse | SCBT | 365956 | NA | 1:50 ON |
| Calbindin | mouse | SCBT | 74462 | NA | 1:50 ON |
| PKC-α | mouse | SCBT | 8393 | NA | 1:50 ON |
| CD11b | rabbit | Abcam | 133357 | NA | 1:200 ON |
| Iba 1 | goat | Abcam | 5076 | NA | 1:800 ON |
| GFAP | mouse | SCBT | 33673 | NA | 1:1000 ON |
| GFAP | rabbit | DAKO | Z0334 | NA | 1:1000 ON |
| RBPMS | mouse | Novus | NBP2-03905 | NA | 1:50 ON |
| RBPMS | rabbit | PhosphoSolutions | 1830-RBPMS | NA | 1:100 ON |
NA= not applicable, ON= overnight