Fig. 5.

Combination treatment with 6-thio-dG and Gamitrinib triggers death selectively in NRAS-mutant tumor cells. a–e NRAS-WT/BRAF-WT melanoma cells (a), NRAS-WT/BRAF-mut melanoma cells (b), NRAS-mutant glioblastoma cells (c), NRAS-WT/KRAS-mutant colon cancer cells (d), and NRAS-WT/KRAS-mutant lung cancer cells (e) were treated with 1 μM 6-thio-dG for 5 days. At day 5, culture medium was replaced and cells treated with 1 μM 6-thio-dG plus 5 μM Gam for two more days. Cells were stained with Annexin V and PI and percent cell death was determined by FACS. Data represent average of three independent experiments ± SEM; p-values were calculated using unpaired Student’s t-test; *p < 0.05; **p < 0.001