Fig. 1. The high expression of HDAC6 was associated with poor prognosis in ESCC specimens, and ACY-1215-inhibited tumor cell proliferation.

a The optimal cutoff values for HDAC6-related mRNA levels were determined and Kaplan–Meier survival curves for patients with ESCC by HDAC6-relatived mRNA levels. b ACY-1215 treatment significantly inhibited cellular proliferation that was dose- and time-dependent manner in EC109 (IC50: 46 μM), KYSE150 (IC50: 57 μM), TE-1 (IC50: 45 μM) and TE-13 (IC50: 37 μM) cells. c The relative levels of HDAC6 expression were higher in all ESCC cell lines than that of the HUVEC cell. d ACY-1215 inhibited cellular proliferation in HUVEC is significantly less than EC109 and TE-1 cells (24 h). The results are shown as the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001