Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 26;8:11255. doi: 10.1038/s41598-018-29308-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Pretreatment with nocodazole enhance IH, GN and Aca-induced apoptosis in MDA-MB-231 cells. MDA-MB-231 cells were pretreated with 100 ng/ml nocodazole for 12 h, followed by treatment with or without 30 μM IH, 20 μM GN and 100 μM Aca for 24 h. (A,B) Cells were stained with cell cycle staining solution and analyzed using a flow cytometer. The percentage of cells in each phase is showed as mean ± S.D. for three independent experiments (^n.sP > 0.05, ^*P < 0.05 or ^**P < 0.01). (C,D) Cells were stained with Annexin V/PI, and the percentage of apoptotic cells were determined using flow cytometry. Data was showed as mean ± S.D (^**P < 0.01) for three independent experiments. (E) Cell lysates were prepared and analyzed by western blotting using antibodies against PARP1, pro-caspase-3 and cleaved-caspase 3 (C-Caspase 3).