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. 2018 Jul 26;8:11250. doi: 10.1038/s41598-018-29258-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ETEC CFA/I fimbriae bind to the CHO-Le^a cells. Immunofluorescence staining of (a) CHO-Le^a and (b) CHO-Le^b cells infected with TOP10-CFA/I. Infected cell lines were stained with anti-CFA/I antibody (Alexa Fluor 488, green), Le^a antibody (Alexa Fluor 647, purple), Le^b antibody (Texas Red, purple) and host nuclei stain (DAPI, Blue). All images are taken at x40 magnification. (c) TOP10-CFA/I bacteria adhere to the CHO-Le^a cell line more than to CHO-Le^b, CHO-CP55 and CHO-K1 cells. Graphs represent the percentage of TOP10-CFA/I bacteria binding to each of the cell lines, as measured by quantifiable immunofluorescence analysis. Statistical analysis was performed using ANOVA with Dunn’s multiple comparisons test. **Indicates P = 0.0065, ****Indicates P = <0.0001. Data presented as Mean and SEM of at least three independent experiments.