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. 2018 Jul 26;8:11250. doi: 10.1038/s41598-018-29258-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ETEC CFA/I and other type 5 pili family members bind to CHO-Le^a cells. (a) ETEC expressing CFA/I⁺ ST⁺/LT⁺ (b) ETEC expressing CS4⁺, ST⁻/LT⁻ (c) ETEC expressing CS14⁺, STh⁺/LT⁻ (d) ETEC expressing CS1⁺, ST⁻/LT⁻ (e) ETEC expressing CS2⁺, ST⁻/LT⁻, and (f) ETEC expressing CS17⁺, ST⁻/LT⁺ and their binding to CHO-Le^a, CHO-Le^b, CHO-CP55 and CHO-K1 cells. Graphs represent the percentage of bacteria binding to each of the cell lines, as measured by quantifiable immunofluorescence analysis. Statistical analysis was performed using ANOVA with Dunn’s multiple comparisons test. **Indicates P = <0.0040, ***Indicates P = 0.0002, ****Indicates P = <0.0001. Data represent the Mean and SEM of two independent experiments.