Fig. 7. ERK5 protein inversely correlates with miR-143 expression, interferes with granulocytic differentiation, and is relevant for myeloid cell survival.

a Western blot for ERK5 protein in G-CSF-treated CD34⁺ HSPCs at indicated time points. The western blot pictures represent one example of three independent experiments. Numbers below the blots represent the mean ratio to the respective controls. b Western blot for phosphorylated ERK5 and ERK5 protein in K562-C/EBPα-ER cells 48 h after β-estradiol treatment. The western blot pictures represent one example of three independent experiments. Numbers below the blots represent the ratio to the respective controls. c Western blot for ERK5 protein (upper panel) and qPCR for miR-143 expression (lower panel) in primary AML patient samples. GAPDH was used for normalization. The western blot pictures represent one example of three technical replicates. For AML patient samples the ratios were divided by the mean of all samples. The bars in the diagram (lower panel) summarize the mean ± SD of three technical replicates. d Transient overexpression of ERK5 in K562-C/EBPα-ER followed by β-estradiol treatment. FACS analysis for CD11b 48 h after transfection. Numbers in dot blots indicate percentage of CD11b⁺ cells. The bars in the diagram summarize the mean ± SD of three independent experiments. P values are relative to control. **P ≤ 0.01, unpaired two-tailed t-tests. e FACS analysis of AnnexinV-stained K562-C/EBPα-ER cells overexpressing ERK5 or control. The bars represent the mean ± SD of three independent experiments. P values are relative to control. *P < 0.05, unpaired two-tailed t-tests