Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 26;50(7):83. doi: 10.1038/s12276-018-0121-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a To confirm a physical interaction between PPP2R5A, the regulatory subunit of PP2A, and HDAC2 in NRVCs, primary cultured cardiomyocytes, 2 mg of protein was immunoprecipitated with an anti-PPP2R5A antibody, and the HDAC2 interaction was visualized with an anti-HDAC2 antibody. b–d The catalytic subunit of PP2A, PPP2CA, also bound to HDAC2. To check the interaction in H9c2 cells, a cardiomyoblast cell line, HDAC2-V5 and PPP2CA-myc were transiently transfected, and cells were lysed with 1% NP-40 lysis buffer. Immunoprecipitation was performed with anti-V5 antibody followed by immunoblotting with anti-Myc antibody. c To check the endogenous interaction in NRVCs, primary cultured cardiomyocytes were lysed in 1% NP-40 lysis buffer. Immunoprecipitation was performed with anti-HDAC2 antibody followed by immunoblotting with anti-PPP2CA antibody. d PPP2CA physically interacted with HDAC2 in mouse heart. Two milligrams of heart lysates were immunoprecipitated with anti-PPP2CA. HDAC2 successfully precipitated with PPP2CA