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. 2018 Jul 26;50(7):83. doi: 10.1038/s12276-018-0121-2

Fig. 3. PPP2CA dissociates from HDAC2 in response to hypertrophic stimuli.

Fig. 3

The binding of PPP2CA to HDAC2 was reduced by hypertrophic stresses (a–c). In cardiomyocytes stimulated with 20 μmol/L PE, the interaction between HDAC2 and PPP2CA was decreased (a). Cardiac hypertrophy induced either by aortic banding (AoB) (b) or by isoproterenol (ISP, 30 mg/kg/day), a beta-adrenergic agonist, also provoked the dissociation of PPP2CA from HDAC2 (c). d To assess whether the hypertrophic stress-induced dissociation is HDAC2 phosphorylation-dependent CK2α1, an HDAC2 kinase was added to the immunoprecipitation assay. HDAC2 phosphorylation also detached PPP2CA from HDAC2. Data are presented as the mean ± SEM. **p < 0.01. White numbers in each bar denote the independent set used for densitometer analysis