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. 2018 Jul 26;50(7):83. doi: 10.1038/s12276-018-0121-2

Fig. 6. PPP2CA targets S394 phosphorylation of HDAC2 to modulate its hypertrophic signal.

Fig. 6

a, b Cell size measurements were performed in NRVCs infected with either Ad-HDAC2 wild-type (WT) or Ad-HDAC2 SE, phosphomimic mutant (SE indicates HDAC2 S394E). Both Ad-HDAC2 WT and Ad-HDAC2 SE successfully induced cardiomyocyte hypertrophy (b, 3rd and 5th group). Ad-PPP2CA abolished Ad-HDAC2 WT-driven cell enlargement. Ad-PPP2CA, however, failed to do so when Ad-HDAC2 SE was infected (b). c Quantification of HPG incorporation. NRVCs were infected with Ad-HDAC2 WT, SE, or Ad-PPP2CA for 48 h. Similar to 5 A and 5B, Ad-PPP2CA failed to suppress Ad-HDAC2 SE-driven cardiomyocyte hypertrophy. d, e OA-mediated hypertrophy was significantly attenuated by infection of Ad-HDAC2 SA, a phospho-resistant mutant of HDAC2 (SA indicates HDAC2 S394A). OA itself induced cardiomyocyte hypertrophy, which was significantly blunted when Ad-HDAC2 SA was infected. PPP2CA targets HDAC2 S394 phosphorylation for its negative regulation of cardiac hypertrophy. White solid bars depict 15 μm. @ indicates p < 0.05. @@ means p < 0.01. *** and @@@ indicate p < 0.001. NS not significant