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. 2018 Jul 20;9:726. doi: 10.3389/fphar.2018.00726

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Copyright © 2018 Lee, Kim, Choi, Choi, Jeong, Park, Choi and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Serum IgG levels, phenotypes of T cells in the lymph nodes, and cytokine expression in the joints of collagen-induced arthritis (CIA) mice. (A) Total serum IgG levels were measured by ELISA. (B) Inguinal lymph nodes were collected from each mouse, and the numbers of CD4⁺IFN-γ⁺ and CD4⁺IL-17A⁺ isolated single cells were detected using FACSCalibur flow cytometry. (C) The joints were excised, total RNA was isolated, and qPCR gene expression analysis was performed. The data are presented as the mean ± SD of five determinations. ^∗p < 0.05, significantly different from untreated CIA mice. 4-HMC, 4-(hydroxymethyl)catechol; Dexa, dexamethasone.