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SNPs and respective primer sequences used for Kafkas-Geo HRM-SNP-assays.
| SNP | Position* | SNP state | Primer forward/reverse (5′-3′) | Reference |
|---|---|---|---|---|
| Kafkas-Geo 2 | 127,121 | A/G | CGGCGAGCAAATGTATGAGTTC/ CTTAGAAACACCACGGAAATCACG | This work |
| Kafkas-Geo 1 | 4,385,818 | T/C | CAATCCGCGCAACCTACAC/ GATCGAGCGCAGTCTTGTG | This work |
| Kafkas-Geo 3 (A.Br.29) | 3,960,657 | G/A | GCCGTTCTACATGCAAAGTATTCC/ ATCGTACTTATTGGTGGTACTGC | 10 |
*In relation to the Ames Ancestor reference genome (NC_007530).
