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. 2018 Jul 20;12:150. doi: 10.3389/fnbeh.2018.00150

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Copyright © 2018 Algamal, Ojo, Lungmus, Muza, Cammarata, Owens, Mouzon, Diamond, Mullan and Crawford.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of RUS on bodyweight gain and anxiety-like behavior. During the 21-day stress paradigm, stressed mice showed a reduction in body weight gain when compared to control mice (A). Growth rate was significantly lower only during and after the first week of stress (day 7) (B). Exposure to stress increased anxiety-like behavior at the acute timepoint as evident by reduced center zone entries per a 3-min time bin (C) and in the total 15 min (D) in the open field test. No significant changes were observed in the EPM open arm time (E) and entries (F) at the acute timepoint. Data in (A,B) was analyzed using repeated measures Two-Way ANOVA followed by post hoc Tukey’s test (n = 14–17), while data in (C–F) were analyzed using a student t-test (n = 8–9). Asterisks denote statistical significance as follows: ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001.